Effects of Pulsed Light on Mycelium Growth and Conidiation in Aspergillus oryzae
杂志:Fermentation-basel
出版年月:2023-7
摘要(英译中):了解米曲霉的光响应机制对酿造和余料处理行业制定有效的光调控方案至关重要。虽然已知连续光照会限制米曲霉的生长,但米曲霉对含光照期的光照的敏感性知之甚少。在这项研究中,我们使用脉冲波调制(PWM)技术产生了9种脉冲蓝光(PBL)处理,这些脉冲蓝光具有不同的峰值光强度和频率。然后我们比较了连续蓝光(CBL)对米曲霉的影响。研究结果表明,GDMCC 3.31米曲霉在光剂量和平均光强恒定不变的情况下,其菌丝在特定峰值强度和频率的PBL下生长速度快,且分生孢子产量多。其中,两种PBL处理(PBL-20_40%_1 Hz和PBL-400_20%_10 kHz)下的菌落直径和分生孢子数分别是CBL下米曲霉的1.13倍和1.22倍。这种不同的反应可能主要归因于米曲霉对自然界光暗循环的适应。此外,还发现峰值光强和频率之间存在交互作用。本研究将脉冲波调制作为影响米曲霉光响应的新因素,并将其应用于发酵光调节方法的开发中。
摘要图:
Figure 3. Colony diameters upon PBL and CBL treatments. Subfigures (a), (b), and (c) are the results of the mycelium growth under PBL with different peak intensities if the frequency was held constant. Subfigures (d), (e), and (f) are the results of the mycelium growth under PBL with different frequencies if the peak intensity was kept unchanged. The symbols *, **, ***, and **** in the figure represent P<0.05, P<0.01, P<0.001, and P<0.0001, respectively. The label PL-100_80%_1 Hz represents a PBL with a peak intensity of 100 μmol×m-2s-1, a duty ratio of 80%, and a frequency of 1 Hz. Other labels are shown in a similar way.